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molecularmandy

Time to count my cells, so I can plate them for treatment tomorrow 🙃 . I count my cells using trypan blue and a hemocytometer. Trypan blue is used to measure viability through an exclusion test. This test is based on the fact that dye is not taken up by viable cells but dead cells have a permeable membrane and can uptake the dye. . Using the hemocytometer, the cells can be counted. The trypan blue cell suspension is put in the chambers of the hemocytometer, which have grid lines so that cells can be counted in each defined area. This allows for an accurate counting of your cell suspension 🤓

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mylifeinthelab

Marvin, even though you’re about a million years old 🧓🏼, you take nearly an hour to make my cDNA ⏳, and you think “hold at ❄️4 degrees Celsius❄️” means “go straight to ☀️room temperature☀️”, you’ve never messed up my PCR 💕 (which a fancy new thermonuclear did this week by reprogramming itself mid-PCR 🤬). I will never doubt you again! 🙏 #ThermocyclerAppreciation #JustPCRthings

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eyedaisyshu

Check out the Insta stories of @sydney_science tomorrow to see a day in the life of me as a PhD student at @sydney_uni 👀👩‍🔬 #instagramtakeover

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molecularmandy

My favourite part of the experiment is the end 👩🏼‍🔬Where I get to put the plate in the plate reader and wait for the results 📊 . . The plate reader or microplate reader has the capability of detecting absorbance. This means, the light source illuminates the samples using a specific wavelength and a light detector on the other side will detect how much of the initial light is transmitted through the sample. The amount of light transmitted through the sample can then be valued and related to specific concentrations (depending on the assay performed).

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mylifeinthelab

Four pretty amazing people became doctors today! #PhD (would you just look at those smiles!!)

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eyedaisyshu

So lucky to be at such a beautiful university 😍 @sydney_uni #usyd #sydneyuni

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mylifeinthelab

This never fails to make me laugh 😂😭 #squishedboy #PCRStillWorkedThough

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eyedaisyshu

The best thing about conferences are the goody bags! Here’s me giving out some for the annual @boschyounginvestigators symposium 😊🙌 #byi2018

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molecularmandy

So those cute little membrane worked very well. Spending the night with ImageJ doing some densitometry on all these little spots in order to determine any differences in protein expression between treated and non-treated cells 🤓🔬

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eyedaisyshu

Deep in discussion at the annual @boschyounginvestigators symposium #byi2018

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dailydoseofchemistry

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molecularmandy

Update: these tiny little membranes are so cute! 🥰 Currently incubating them with my samples overnight so the proteins in my cell samples can bind to the 40 different spots on each of them 🧪

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eyedaisyshu

Presented my poster yesterday at the 18th Bosch Young Investigator Annual Symposium. It’s about the role of growth factors in wound healing of the cornea, the transparent front tissue in our eyes 👀 Thanks to everyone for coming along to check it out! #byi2018 #posterpresentation

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molecularmandy

Thawing some new little buddies (aka cells). For cell culture, new vials of cryo-preserved cells have to be thawed from liquid nitrogen . . Liquid nitrogen preserves the cells at a very cold temperature (-196 degrees celsius).The cool temperature stops the cells enzymatic activity . . In order for the cells to freeze in liquid nitrogen without forming ice crystals, freezing media is used. A common additive to media is DMSO (dimethyl sulfoxide) in low concentrations . . Today I’m thawing THP-1 cells, which are a monocytic cell line, derived from a person with monocytic leukemia 🔬

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labpro_inc

Come visit us at the Silicon Valley’s SMTA conference, booth #72. #SMTA offers exclusive access to local and global communities of experts as well as accumulated research and training materials from thousands of companies dedicated to advancing the #electronics industry. 👨🏼‍🔬👩🏻‍🔬 * * * * * * #lab #instascience #labmania #youinthelab #LaboratoryDistributor #LaboratorySupplies #LabSupplies #LabDistributor #Chemistry #Physics #Biology #Science #Microscope #Scientificlabsupplies #ScienceSupplies #laboratory #laboratoryglassware #Labsupplier #SanJose #Paloalto #Bayarea #Sunnyvale #Sanfrancisco #Santaclara #LabPro #LabProinc #IAmALabPro

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eyedaisyshu

‪Excited to talk tomorrow at the Bosch Young Investigators Annual Symposium about the role of growth factors in cataract formation! It will be at Charles Perkins Centre Auditorium @sydney_uni @sydney_health @sydney_science 😊🙌A huge thank you to @HigherEdPR for making this amazing graphic for me #byi2018 The Final Program can be found here bit.ly/2TTbYwn‬ @boschyounginvestigators

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molecularmandy

My PhD project is mainly immunology focused but also involves some cancer research . . I use 96-well plates for some experiments. These plates have 96 wells that will hold your cells for different treatment types (on a small scale) . . This image shows 96 well plates that have been stained with SRB dye (sulforhodamine B). This dye is a bright pink and binds to cellular protein content . . The use of this dye has been used for the toxicity screening of compounds. The bound protein dye can be dissolved in solution in order to record the absorbance. The absorbance reading can then be used to measure cellular protein and be translated into an IC50 value . . In cancer research, a compound’s IC50 value is very informative. It allows us to know how well a compound can kill inhibit the growth of cells. . . So as you can see, we do lots of them👩🏼‍🔬🧪

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biotecbr

Para descontrair! 🤣🙈 ❗️Curta, comente ou deixe uma carinha para nossa página não sair do seu feed. Please like our instagram posts! #instascience #labmania #youinthelab #labs #science #biotechnology #study #biology #brazil #bioscience #study #life #lifescience #ciencia #biologia #biotec #biotecnologia #ciência #brasil #biociencias #quimica #estudo #brasil

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eyedaisyshu

It’s beginning to look a lot like Christmas ❤️💚🌲 #christmas

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eyedaisyshu

There’s a party in the incubator and all the cells are invited 🙋‍♀️✨ #partytime

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eyedaisyshu

Some Monday motivation for you all! 😊🙌🙌 Let’s make this week an awesome one! 🤘

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eyedaisyshu

What time is it? PCR o’clock 😂🙌 #qpcr #realtime #gettingreal

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dyana_bunny

Coshitas 👩🏻‍🔬👨🏻‍🔬 #qfbt #uvm #uvmslp #youinthelab #meinthelab

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eyedaisyshu

Self-care ✅✅✅ As much as it is important to get your experiments and writing done, don’t forget to take some time out to have fun and explore new places! Yesterday I went hiking and hit the beach and found these gorgeous pools in the shape of a figure 8 🎱😍

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eyedaisyshu

I’m spinning around, move outta my way 🎵🎶 Love using magnetic fleas to mix buffers! 😂🤘🤘

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ikaworldwide

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biotecbr

Quando trabalhamos com amor, somos felizes e realizados . Obrigada, @mellany When we love what we do, we feel happy and fulfilled. . #estudo #youinthelab #ciencia #pesquisa #pesquisadores #semic #iniciacaocienrifica #banner #lovemywork #vidadeestudante #ufma

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eyedaisyshu

So many samples, and so much finger cramping 😭 #datpipettinglife

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mylifeinthelab

My lab = pub trivia dream team!! 🙌💫 (even though we came 3rd 😅) . Does you get together and do fun things with your team outside the lab?

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eyedaisyshu

Is it just me or are mini tubes you find in the lab just the most adorable thing ever? 😍😍

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labpro_inc

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eyedaisyshu

Insane in the PVDF membrane 🤪 #westernblot

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eyedaisyshu

A coffee a day is what keeps me going 🤓🙌☕️💤🌞 #butfirstcoffee

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eyedaisyshu

I like my transfers cold 🥶❄️ My tip for western blotting is to do the transfer step packed with ice so the membrane doesn’t burn, especially when you need to transfer the big proteins that take longer 😊👌 #westernblot

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cajapetri

••• Give Live Back To Microbiology •••••••••••••••••••••••• Esto fue una observación de levaduras de pan con una tinción simple :B •••••••••••••••••••••••• #VSCO #vscocool #vscocam #vscogallery #vscogrid #vscocamera #vscogrid #Instagram #Instagood #Instacam #instagrammer #instagrid #instagallery #instacool #Canon #CanonMexicana #CanonMexico #Canonistas #igers #igersmexico #igersveracruz #igersorizaba #igerscreativos #mexigers #Beginnersmx #mextagram #CreativosMx #communityfirst #YouInTheLab #AsMicrobiology •••••••••••••••••••••••• Muchas gracias amixes :3 Pronto más fotos nuevas :B jijiji

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eyedaisyshu

Making gels 💚 is rad, it’s Bio-rad 🤙🤙🤙 @bioradlabs

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eyedaisyshu

Science mode activated! Thanks @maryflokis for capturing this shot of us working on our immunofluorescence @lensresearchlab

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eyedaisyshu

Homebound to Sydney ✈️ Had such an amazing time at #Academy18 @aaopt meeting in San Antonio but it’s time to say goodbye to this beautiful and lively city to return home to Sydney and finish my PhD!

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eyedaisyshu

Visited the Alamo while we were in San Antonio for the #academy18 @aaopt and got a history lesson in how medicine was practiced back in the day! Wow we really have come a long way! 😱🙌

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eyedaisyshu

Had such an amazing time at #academy18 in San Antonio! Love the artwork “Through our eyes” by the @aaopt Foundation to capture the eyes of optometrists and eye researchers around the world and share in our vision to promote eye care to everyone 😊👀💕

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mylifeinthelab

Beautiful confocal of a heterogeneous population of stressed out lens epithelial cells by our very own @impermanant_surrounds 👏

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eyedaisyshu

When you’re feet hurt from conferencing all day and you decide to just sit on the carpet 😂👌 #academy18 @aaopt

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eyedaisyshu

Made some new friends at #academy18 @aaopt in San Antonio, Texas 😊💕

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mylifeinthelab

Ancient manual cell counter! My new favourite thing since my (not so) trusty automatic cell counter broke yesterday 😭😅 #myhero

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eyedaisyshu

At the International Reception at #academy18 @aaopt in San Antonio, Texas! Got to catch up with my fellow Aussie friends Debarun and Terry!

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eyedaisyshu

Lots of amazing trade displays at the #Academy18 exhibition hall @aaopt

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mylifeinthelab

Happy #saturdayinthelab ✌️

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mylifeinthelab

When it’s Saturday but you still have to go look after your cells 😭😭😭

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eyedaisyshu

Cool phone camera attachment to capture images of the eye on the slit lamp microscope 🔬👀 #academy18 @aaopt #optometry #eyes

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eyedaisyshu

‪Playing the “Hoop it up” 2020 challenge at #Academy18 The aim is to get 2020 plays. We’re at 1195 now! If we reach the goal, @AlconEyeCare will donate $25000 to @aaopt AAOF foundation! 😊 🏀 👀 #optometry

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eyedaisyshu

Ezell Fellows 😊👀🙌 #Academy18 @aaopt

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eyedaisyshu

Last night at the AAOF function at #Academy18 @aaopt #texas #optometry

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eyedaisyshu

Made some amazing new friends at #Academy18 @aaopt meeting in San Antonio, Texas 😊🙌✈️ #optometry

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eyedaisyshu

Me presenting my talk today on a novel mechanism in cataract formation at #Academy18 @aaopt Thanks for the great questions! 😊👀🙌💕👌

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eyedaisyshu

A packed session at #Academy18 at the “Do you want steroids with that?” by the charismatic and entertaining speaker Bruce Onofrey 😊🙌🙌🙌 @aaopt 👀👌#optometry #steroids

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eyedaisyshu

Last year at #Academy17 I presented a poster on my work on EGFR signaling in cataract formation and this year at #Academy18 I will be presenting a talk that builds on these findings! @aaopt #progress 😊🙌

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dailydoseofchemistry

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mylifeinthelab

Thanks for the rad new labcoats @thermofisherscientific! How cool are black labcoats!! 🖤🤘#PrepOn

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ikaworldwide

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stefanthechemist

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eyedaisyshu

Goodbye Sydney! ✈️😊 Off to San Antonio, Texas for @aaopt #Academy18

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eyedaisyshu

We have lift off! 🚀 Excited to be heading to San Antonio, Texas for #Academy18 @aaopt annual meeting 😊✈️ I’ll be presenting a talk tomorrow in the “Animal Models of Ocular Disease” session at 1pm #liftoff

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