NUTRIENT AGAR MEDIUM
-Nutrient Agar is used as a general purpose medium for the cultivation of less fastidious microorganisms, can be enriched with blood or other biological fluids.
-Nutrient media are basic culture media used for maintaining microorganisms, cultivating fastidious organisms by enriching with serum or blood and are also used for purity checking prior to biochemical or serological testing (6,7).
-Nutrient Agar is ideal for demonstration and teaching purposes where a more prolonged survival of cultures at ambient temperature is often required without risk of overgrowth that can occur with more nutritious substrate. This relatively simple formula has been retained and is still widely used in the microbiological examination of variety of materials and is also recommended by standard methods. It is one of the several non-selective media useful in routine cultivation of microorganisms (1,2). It can be used for the cultivation and enumeration of bacteria which are not particularly fastidious.
-Addition of different biological fluids such as horse or sheep blood, serum, egg yolk etc. makes it suitable for the cultivation of related fastidious organisms.
-Peptone, HM peptone B and yeast extract provide the necessary nitrogen compounds, carbon, vitamins and also some trace ingredients necessary for the growth of bacteria.
-Sodium chloride maintains the osmotic equilibrium of the medium.
-Appearance: cream to yellow homogeneous free flowing powder.
-Organisms grown on this media: Streptococcus pyogenes, Salmonella typhi, S aureus, Pseudomonas, E coli, & many more..
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